Improved method of polymer purification

An improved method for the purification of staphylococcal nuclease.

Commercial staphylococcal nuclease (Foggi strain) containing small amounts of contaminating protein can be purified by chromatography on phosphorylated cellulose columns. The purified enzyme is free of acid and alkaline phosphatase activities. It is homogeneous by polyacrylamide gel electroptfresis, and antigenically pure by immunodiffusion. The E” 1 Cm of the pure enzyme at neutral pH is 9.3 a...

High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication

Pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. This bacterium is motile via a single polar flagellum made of polymerized flagellin subunits differentiated into two major serotypes: A and B. flagellin plays an important role as a virulence factor in the adhesion, colonization and invasion of P. aer...

Affinity partitioning. A method for purification of proteins using specific polymer-ligands in aqueous polymer two-phase systems.

We describe a method, called affinity partitioning, for the purification of proteins containing specific ligand binding receptor sites. This method adds specificity to the procedures for protein purification with aqueous polymer two-phase systems by introduction of a polymer derivative, coupled to an appropriate ligand. The addition of a polymer-ligand that partitions predominantly into one pha...

A NOVEL METHOD OF PURIFICATION OF SPECIFIC HYDATID CYST ANTIGEN

Hydatid cyst fluid has been used as a source of antigen for the serodiagnosis of Echinococcus granulosus infection. Due to cross-reactions with antigens shared by other helminthes, the specific antigen from hydatid cyst fluid was purified by many workers. We used a relatively simple technique for purification of specific antigen from sheep hydatid cyst fluid. Isopycnic ultracentrifugation w...

Polymer Nanocomposite Hydrogels for Water Purification